Flow Cytometry Suite

Overview

The CDI offers a modern Flow Cytometry Core Facility that includes high parameter fluorescent activated cell sorting (FACS) and flow cytometry instruments and services. The Flow Cytometry Core Facility lends its expertise in their capabilities to the CDI community and provides consultation on experimental design and fluorescent panel development, as well as in-depth training to allow unsupervised usage. The CDI Flow Cytometry Core Facility is focused on providing top-of-the-line instrumentation that will maximize scientific impact for all of its users. We communicate with our scientific community and partners to ensure that we continually deliver improvements and cutting edge, reliable services. The resources of the core facility are available to all investigators at the CDI and its affiliated institutions.

CDI is part of the National Cancer Institute-designated Georgetown Lombardi Comprehensive Cancer Center, and additional flow cytometry resources are available and can be accessed here.

Instrumentation and Services

Technical Services offered:

  • Guidance in experimental methodologies and design
  • Technical assistance for grant proposals and scientific manuscripts
  • Assay development to fit users’ research-specific needs
  • Instrumentation and analysis software training

Cytometry Equipment available:

  • BD LSRFortessa flow cytometer analyzer configured for four LASER (405nm, 488nm, 561nm,640 nm), 16 color analysis (BD LSRFortessa specifications)
  • BD FACSymphony A3 flow cytometer analyzer configured for five LASER (355nm, 405nm, 488nm, 561nm, 637nm), 28 color analysis (BD FACSymphony A3 specifications)
  • BD FACSAria III cell sorter configured for five LASER (355nm, 405nm, 488nm, 561nm, 640nm), 15 color analysis (BD FACS Aria III specifications). Sorting capabilities include four-way collection tubes, variable plate sizes, and single cell 96 well plates. ARIA III sorter is housed in a high containment BioBUBBLE benchtop enclosure
  • BD FACSMelody cell sorter configured for three LASER (488nm, 561nm, 640nm), 8 color analysis (BD FACSMelody specifications). Sorting capabilities include two-way collection tubes, variable plate sizes, and single cell 96 well plates. Melody sorter is housed in a BSL2 biosafety cabinet

Analysis workstation:

A stand-alone workstation is available. The workstation is equipped with Flowjo

Faculty and Staff

  • Wenshan Tsao, M.S. (Manager) manages day-to-day operations of the FCSR. Wenshan received her graduate training in Biotechnology from the National Dong Hwa University and has 7 years of experience in cell analysis and cell sorting. Tsao is available for instrument training, cytometric analysis, cell sorting, experimental consultation and troubleshooting

Fee and scheduling

Fees

Please contact Wenshan (wenshan.tsao@hmh-cdi.org) for current fee

Scheduling and billing

Investigators must schedule with Tsao (wenshan.tsao@hmh-cdi.org) for their mandatory initial training.

Investigators, once they have completed the mandatory initial training for a given instrument, are then free to book time on the instrument through the Idea Elan portal.

Optical Configuration - Instruments

Core Resources

Core facilities include high-end imaging, mass spectrometry, gene editing, cell sorting, flow cytometry, tissue banks and biocontainment. A high-tech auditorium, Board Room and video conferencing center serve as venues for both internal and external speakers to present their research to CDI scientists, professors and medical students. An additional ~60,000 GSF of lab space is available for expansion of the research enterprise.