Research in the Zilberberg Lab
Microfluidic approach for the development of a three-dimensional bone marrow microenvironment model to test personalized multiple myeloma treatments
Through this project, we aim to: (1) develop an off-the-shelf technology that enables reconstruction of bone marrow and microenvironmental factors regulating survival and proliferation of multiple myeloma tumor cells present in bone marrow biospecimens, and (2) demonstrate the utility of the bone marrow biospecimen 3D-culturing platform to assess drug treatment strategies for multiple myeloma.
Ex vivo culture platform validation for preservation of patient derived multiple myeloma cells
The goal of this project is to develop and validate an ex vivo multiple myeloma culture platform that utilizes patient biospecimens for screening patient-specific treatment options.
Biomimetic assembly of microphysiological lacunocanalicular network
The goals of the project are to: (1) investigate the effects of biphasic calcium phosphate and hydroxyapatite nanoparticles on extracellular matrix production by human primary osteocytes and remodeling of their lacunocanalicular space, and (2) reproduce the physiological role of the reconstructed osteocyte network in incorporating neighboring osteoblasts into the cellular network, and regulating the spatiotemporal transition of the osteoblasts to osteocytes as mechanisms by which new bone tissue is formed during homeostatic bone remodeling.
Mitigating adhesion between myeloma cells and the tumor microenvironment to abrogate drug resistance
The goal of this project is to determine the feasibility of manipulating patient-derived multiple myeloma cells’ adhesive interactions with the endosteal niche to abrogate drug resistance in multiple myeloma.
We thank the following agencies for supporting our current and past research:
Q. Sun, S. Choudhary, C. Mannion, Y. Kissin, J. Zilberberg*, and W.Y. Lee*, “Ex Vivo Replication of Phenotypic Functions of Human Primary Osteocytes through 3D Bone Tissue Construction,” Bone (In Press).
Q. Sun, S. Choudhary, C. Mannion, Y. Kissin, J. Zilberberg*, and W.Y. Lee*, “Ex Vivo Construction of Human Primary 3D-Networked Osteocytes,” Bone, 105, 245-252 (2017).
S. Choudhary, Q. Sun, C. Mannion, Y. Kissin, J. Zilberberg*, and W.Y. Lee*, “Hypoxic 3D Cellular Network Construction Preserves Ex vivo the phenotype of Primary Human Osteocytes,” Tissue Engineering A DOI: 10.1089/ten.tea. 2017.0103.
Zhang W, Lee WY*, Zilberberg J*. Tissue Engineering Platforms to Replicate the Tumor Microenvironment of Multiple Myeloma. Methods Mol Biol. 2017;1513:171-191.
Q. Sun, Y. Gu, W. Zhang, L. Dziopa, J. Zilberberg*, and W.Y. Lee*, “Ex Vivo 3D Osteocyte Network Construction with Primary Murine Bone Cells,” Bone Research, 3, 152-163.3 (2015).
W. Zhang, Y. Gu, Q. Sun, D.S. Siegel, P. Tolias, Z. Yang, W.Y. Lee*, and J. Zilberberg*, “Ex Vivo Maintenance of Primary Human Multiple Myeloma Cells through the Optimization of the Osteoblastic Niche,” PLoS ONE 10(5): e0125995 (2015).
Y. Gu, W. Zhang, Q. Sun, Y. Hao, J. Zilberberg*, and W.Y. Lee*. Microbeads-Guided Reconstruction of 3D Osteocyte Network during Microfluidic Perfusion Culture. Journal of materials chemistry. B, Materials for biology and medicine 3, 3625-3633, doi:10.1039/C5TB00421G (2015).
W. Zhang, Y. Gu, Y. Hao, Q. Sun, K. Konior, H. Wang, J. Zilberberg, and W.Y. Lee, “Well Plate-Based Perfusion Culture Device for Tissue and Tumor Microenvironment Replication,” Lab-on-a-Chip, 15, 2854-2863 (2015).